Dementia is a illness that causes reminiscence and lack of judgment. There is not any fundamental therapy for individuals who develop the scientific image. Years of scientific analysis have confirmed that protein aggregation happens in lots of neurodegenerative illnesses, together with dementia. This reveals that the prevalence of this aggregation is carefully linked to illnesses, improvement and illness development. Aggregation happens when regular protein kinds irregular buildings for sure causes and accumulates within the cell, with every illness having a distinct aggregating protein. It is thought that the protein known as “α-synuclein” kinds irregular buildings in Parkinson’s, Lewy physique dementia and a number of system atrophy within the affected person’s mind.
The α-synuclein aggregation assay equipment mimics the intracellular aggregation of “α-synuclein”, which permits in vitro screening of medicine.
α-Synuclein Kit composition
|Components||quantity||quantity||storage4 ° Cdo not freeze|
|pCMV-SNCA (α – Synuclein Expression plasmid vector, Red cap) (conc. 1.25 μg / μL)||32 μL||1 vial|
|pCMV-NC (Negative management vector, Green cap)(conc. 1.25 μg / μL)||5 μL||1 vial|
|pCMV-dGFP (dGFP Expression plasmid vector, blue cap)(conc. 1.25 μg / μL)||5 μL||1 vial|
|20 mM Tris-HCl buffer (pH7.4)||10 mL||1 bottle|
|F-αSyn (α – Synuclein Fibrils, Yellow cap) (conc. 1 μg / μL)||32 μL||1 vial|
|Multifectam (gene transfection reagent)||0.33 mg||1 bottle|
α-Synuclein Aggregation Assay Kits
|α-Synuclein Aggregation Assay Kit||CSR-SYN01-COS||1 KIT (300 checks)|
|Amyloid fluorescent staining equipment||CSR-SYN02-COS||1 KIT (100 checks)|
|α-synuclein fibrils||CSR-SYN03-COS||0.1 MG|
|α-synuclein, recombinant E. coli||CSR-SYN04-COS||1 MG|
|α-synuclein, recombinant E. coli||CSR-SYN04-COS||0.1 MG|
Alpha-synuclein, a 140-amino acid protein that’s abundantly expressed in presynaptic terminals and as a element of intraneuronal or glial inclusions, which is one in every of Parkinson’s (PD), dementia with Lewy our bodies (DLB) and a number of system atrophy (MSA) play a serious function. Alpha-Synuclein is a natively unfolded protein, however fibrillation or conformational change of Alpha-Synuclein is central to the pathogenesis of Alpha-Synucleinopathies. The amino-terminal area of alpha-synuclein consists of seven imperfect repeats, every 11 amino acids lengthy, with the consensus sequence KTKEGV. The repetitions partially overlap with a hydrophobic area (amino acids 61-95). The carboxy-terminal area (amino acids 96-140) is negatively charged.
The antibodies on provide are highly effective instruments for biochemical and IHC analyzes of neurodegenerative illnesses and for evaluating conformational adjustments of alpha-synuclein.
|Anti (ALPHA) Synuclein (1-10)||CAC-TIP-SN-P01||50UL|
|Anti (ALPHA) Synuclein (11-20)||CAC-TIP-SN-P02||50UL|
|Anti (ALPHA) Synuclein (21-30)||CAC-TIP-SN-P03||50UL|
|Anti (ALPHA) Synuclein (31-40)||CAC-TIP-SN-P04||50UL|
|Anti (ALPHA) Synuclein (41-50)||CAC-TIP-SN-P05||50UL|
|Anti (ALPHA) Synuclein (51-60)||CAC-TIP-SN-P06||50UL|
|Anti (ALPHA) Synuclein (61-70)||CAC-TIP-SN-P07||50UL|
|Anti (ALPHA) Synuclein (75-91)||CAC-TIP-SN-P08||50UL|
|Anti (ALPHA) Synuclein (131-140)||CAC-TIP-SN-P09||50UL|
|Anti (ALPHA) Synuclein (9 antibodies)||CAC-TIP-SN-SET||9 * 10UL|
- Airi T. et al. J Biol Chem. 2016 Sep 2; 291 (36): 18675-88
- Masami Masuda, et al. Inhibition of a-synuclein fibril meeting by small molecules: A FEBS Letters (2009) 583, 787-791. PMID 19183551
- Motokuni Yonetani, et al. Conversion of wild-type alpha-synuclein into mutant-type fib A30P mutant. Journal of Biological Chemistry (2009) 284, 7940-7950. PMID 19164293
- Nonaka T. et al. J Biol Chem. 2010 Nov 5; 285 (45): 34885-98